Abstract In recent studies. electronegative multimodal chromatography with Eshmuno HCX was demonstrated to be a highly promising recovery step for direct immunoglobulin G (IgG) capture from undiluted cell culture fluid. In this study. the binding properties of HCX to IgG at different pH/salt combinations were systematically studied. https://parisnaturalfoodes.shop/product-category/goldenseal/